1. GPCR/G Protein Neuronal Signaling Metabolic Enzyme/Protease
  2. Adrenergic Receptor Endogenous Metabolite
  3. Isoprenaline hydrochloride

Isoprenaline hydrochloride  (Synonyms: 盐酸异丙肾上腺素; Isoproterenol hydrochloride)

目录号: HY-B0468 纯度: 99.86%
COA 产品使用指南

Isoprenaline (Isoproterenol) hydrochloride 是一种非选择性的 β-肾上腺素受体激动剂,具有口服活性。Isoprenaline 具有强效的外周血管扩张、支气管扩张和心脏刺激活性。Isoprenaline 可用于心动过缓和支气管哮喘的研究。

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Isoprenaline hydrochloride Chemical Structure

Isoprenaline hydrochloride Chemical Structure

CAS No. : 51-30-9

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Customer Review

Other Forms of Isoprenaline hydrochloride:

MCE 顾客使用本产品发表的 51 篇科研文献

WB
RT-PCR

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: Cell Mol Immunol. 2023 Jan 5.  [Abstract]

    Isoprenaline hydrochloride (ISO; 10 μM; 6 h), markedly inhibits SeV- or HSV-1-induced transcription of the IFNB1 and CXCL10 genes in THP1 cells.

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: Cell Mol Immunol. 2023 Jan 5.  [Abstract]

    Isoprenaline hydrochloride (ISO; 10 μM; 0, 3, 6, 9 h) inhibits the phosphorylation of MITAS366, IRF3S386 and STAT1Y701 induced by HSV-1, HSV-2 and HCMV, as well as the phosphorylation of IRF3S386 and STAT1Y701 induced by SeV, EMCV and VSV, in THP1 cells.

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: Food Chem Toxicol. 2023 Feb 17;113670.  [Abstract]

    Isoprenaline hydrochloride (ISO; 10 μM; 6 h) pretreatment markedly restores the ATGL and HSL expressions decreased by 1,3-dichloro-2-propanol (1,3-DCP; 100 μM; 6 h) in HepG2 cells.

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2019 Apr 25;38(1):174.   [Abstract]

    EHD1 stabilizes β2AR. A549 cells are incubated in serum-free 1640 medium for 16 h and then treated with ISO (10 μM) for the indicated times in the presence of cycloheximide (CHX, 20 μg/mL). The cell lysates are analyzed by Western blot.

    Isoprenaline hydrochloride purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2018 Dec;391(12):1373-1385.  [Abstract]

    NRCMs are pre-incubated with PCA (50, 100, and 200 μM) for 1 h followed by 10 μM Isoproterenol (ISO) treatment. Cell surface area is measured by rhodamine-phalloidin staining. The expression of β-MHC is detected by Western blot.

    查看 Adrenergic Receptor 亚型特异性产品:

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Isoprenaline (Isoproterenol) hydrochloride is a non-selective, orally active β-adrenergic receptor agonist. Isoprenaline has potent peripheral vasodilator, bronchodilator, and cardiac stimulating activities. Isoprenaline can be used for the research of bradycardia and bronchial asthma[1][2][3][4][5][6].

    IC50 & Target

    β adrenergic receptor

     

    体外研究
    (In Vitro)

    Isoprenaline (Isoproterenol) hydrochloride (300 nM, 3 min) increases particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 100% in intact rat fat cells[1].
    Isoprenaline inhibits insulin-stimulated glucose transport activity in rat adipocytes. Isoprenaline, in the absence of adenosine, promotes a time-dependent (t1/2 approximately 2 min) decrease in the accessibility of insulin-stimulated cell surface GLUT4 of > 50%, which directly correlated with the observed inhibition of transport activity[2].
    Isoprenaline (5 nM and 10 μM) increases cyclic AMP levels and this effect is potentiated by cilostamide (10 mM), by rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 mM) and by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO)[3].
    Isoprenaline increases the transcriptional activity of Gi alpha-2 gene to 140% of the control value, whereas gene specific hybridization for Gs alpha remains unchanged[4].
    Isoprenaline (20 nM) increases the amplitude of total iK and causes a negative shift of approximately 10 mV in the activation curve for iK, both in the absence and in the presence of 300 nM nisoldipine to block the L-type Ca2+ current[5].
    Isoprenaline (20 nM) increases the spontaneous pacemaker rate of sino-atrial node pacemaker cells by 16% in rabbit isolated pacemaker cells[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Isoprenaline (Isoproterenol) hydrochloride (oral, 0.27-0. 64 μg/kg) is extensively metabolizes by a relatively small number of reactions in dogs[6].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Dogs[1]
    Dosage: 0.27-0. 64 μg/kg
    Administration: oral
    Result: Excreted largely unchanged in urine, only one-third of the radioactivity in urine was in the form of the O-methyl metabolite.
    Showed plasma radioactivity was almost entirely as conjugated isoprenaline and this metabolite accounted for more than 80% of radioactivity in urine.
    . Showed heart rate returned to base-line values when high plasma concentrations.
    Clinical Trial
    分子量

    247.72

    Formula

    C11H18ClNO3

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    盐酸异丙肾上腺素

    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    溶解性数据
    细胞实验: 

    DMSO 中的溶解度 : 80 mg/mL (322.95 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    H2O 中的溶解度 : ≥ 50 mg/mL (201.84 mM)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.0368 mL 20.1841 mL 40.3682 mL
    5 mM 0.8074 mL 4.0368 mL 8.0736 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.40 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.40 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。

    以下溶解方案,请直接配制工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 100 mg/mL (403.68 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.86%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.0368 mL 20.1841 mL 40.3682 mL 100.9204 mL
    5 mM 0.8074 mL 4.0368 mL 8.0736 mL 20.1841 mL
    10 mM 0.4037 mL 2.0184 mL 4.0368 mL 10.0920 mL
    15 mM 0.2691 mL 1.3456 mL 2.6912 mL 6.7280 mL
    20 mM 0.2018 mL 1.0092 mL 2.0184 mL 5.0460 mL
    25 mM 0.1615 mL 0.8074 mL 1.6147 mL 4.0368 mL
    30 mM 0.1346 mL 0.6728 mL 1.3456 mL 3.3640 mL
    40 mM 0.1009 mL 0.5046 mL 1.0092 mL 2.5230 mL
    50 mM 0.0807 mL 0.4037 mL 0.8074 mL 2.0184 mL
    60 mM 0.0673 mL 0.3364 mL 0.6728 mL 1.6820 mL
    80 mM 0.0505 mL 0.2523 mL 0.5046 mL 1.2615 mL
    100 mM 0.0404 mL 0.2018 mL 0.4037 mL 1.0092 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

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    Isoprenaline hydrochloride
    目录号:
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